Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources

Polysaccharide is widely distributed in natural resources, and currently attracts scientists' attention for their various bio-functions including immunomodulatory activity, hypoglycemic hypolipidemic antitumor promoting effects on gastrointestinal function, so on. The purity structure characteristics of different polysaccharides greatly restrict the in-depth study bioactivity mechanism. Therefore, this guideline will display extraction, isolation, purification, structural characterization from resources. extraction methods acid-soluble polysaccharides, water-soluble alkaline soluble are included. Common purification like membrane filtration, alcohol precipitation, column chromatographic separation explained detail. A comprehensive procedure polysaccharide given molecular weight monosaccharide composition to Fourier transform infrared nuclear magnetic resonance analysis. Examples added where necessary help you better understand guideline.